Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus

0584200 - BC 2024 RIV US eng J - Journal Article
Edwards, A. - Njaci, I. - Sarkar, A. - Jiang, Z. - Kaithakottil, G. G. - Moore, C. - Cheema, J. - Stevenson, C. E. M. - Rejzek, M. - Novák, Petr - Vigouroux, M. - Vickers, M. - Wouters, R. H. M. - Paajanen, P. - Steuernagel, B. - Moore, J. D. - Higgins, J. - Swarbreck, D. - Martens, S. - Kim, C. Y. - Weng, J. - Mundree, S. - Kilian, B. - Kumar, S. - Loose, M. - Yant, L. - Macas, Jiří - Wang, T. L. - Martin, C. - Emmrich, P. M. F.
Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus.
Nature Communications. Roč. 14, č. 1 (2023), č. článku 5199. E-ISSN 2041-1723
R&D Projects: GA MŠMT(CZ) LM2018131
Institutional support: RVO:60077344
Keywords : Grass pea (Lathyrus sativus L.) * L. sativus genome * biosynthetic pathway * neurotoxin
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 16.6, year: 2022
Method of publishing: Open access
https://www.nature.com/articles/s41467-023-36503-2

Grass pea (Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a changing climate. The lack of genetic resources and the crop’s association with the disease neurolathyrism have limited the cultivation of grass pea. Here, we present an annotated, long read-based assembly of the 6.5 Gbp L. sativus genome. Using this genome sequence, we have elucidated the biosynthetic pathway leading to the formation of the, β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP). The final reaction of the pathway depends on an interaction between L. sativus acyl-activating enzyme 3 (LsAAE3) and a BAHD-acyltransferase (LsBOS) that form a metabolon activated by CoA to produce β-L-ODAP. This provides valuable insight into the best approaches for developing varieties which produce substantially less toxin.
Permanent Link: https://hdl.handle.net/11104/0352311