Towards highly sensitive glycosylation analysis by CE/LIF and CE-MS

0584033 - ÚIACH 2025 DE eng A - Abstract
Lavická, Jana - Smolková, Denisa - Duša, Filip - Čmelík, Richard - Šesták, Jozef
Towards highly sensitive glycosylation analysis by CE/LIF and CE-MS.
CE Forum 2024. Aalen: Aalen University, 2024. s. 10-10.
[CE Forum 2024. 28.02.2024-29.02.2024, Aalen]
R&D Projects: GA ČR(CZ) GA22-00236S
Institutional support: RVO:68081715
Keywords : capillary electrophoresis * mass spectrometry * laser-induced fluorescence
OECD category: Analytical chemistry

Capillary electrophoresis (CE) is a powerful separation technique that has become widely used in glycosylation analysis. However, due to the low electrophoretic mobility as well as the absence of chromophore or fluorophore in the structure of native glycans, appropriate modification of the saccharide molecule is required prior to electrophoretic separation and detection by either optical methods and/or mass spectrometry (MS). In this work, we present the design, synthesis, and application of novel fluorescent labels that feature fast labeling kinetics, high fluorescence quantum yields, and significantly improved detectability by MS. Novel fluorescent labels based on pyrene, rhodamine and/or BODIPY derivatives with different substituents and modifiable linkers were synthesized and the required modification allowed tuning the fluorescence and MS properties (preferred wavelength and quantum yield, charge). Labeling by different synthesized labels and their comparison with commercially available labels will be demonstrated by analysis of maltooligosaccharides and N-linked glycans by CE with laser-induced fluorescence (CE/LIF) and/or CE-MS.
Permanent Link: https://hdl.handle.net/11104/0352037