Improving the glycoproteomic workflow for the analysis of N-linked glycans by CE/LIF

0584031 - ÚIACH 2025 DE eng A - Abstract
Smolková, Denisa - Bobálová, Janette - Strouhalová, Dana - Čmelík, Richard - Lavická, Jana
Improving the glycoproteomic workflow for the analysis of N-linked glycans by CE/LIF.
CE Forum 2024. Aalen: Aalen University, 2024. s. 18-18.
[CE Forum 2024. 28.02.2024-29.02.2024, Aalen]
R&D Projects: GA ČR(CZ) GA22-00236S
Institutional support: RVO:68081715
Keywords : N-linked glycans * CE/LIF
OECD category: Analytical chemistry

Structural and quantitative changes in N-linked glycans are involved in several human disorders. High-throughput profiling of N-glycans is a key step to elucidate the functions of glycans in biological processes and disease development and to discover new diagnostic biomarkers. In this work, we have optimized a workflow for the analysis of N-linked glycans by CE/LIF. Glycoproteomic protocols differing in enzymatic or digestion procedures (alkylation, gel/solution treatment, purification step, etc.) were evaluated to achieve highly sensitive detection. The released glycans were derivatized with Cascade Blue hydrazide (8-(2-hydrazino-2-oxoethoxy)pyrene-1,3,6-trisulfonic acid trisodium salt, CBH) as a more reactive analogue of 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt (APTS) followed by CE/LIF analysis.
Permanent Link: https://hdl.handle.net/11104/0352035