Combinatorial libraries of bipodal binders of the insulin receptor.

0584024 - ÚOCHB 2025 RIV US eng J - Journal Article
Selicharová, Irena - Fabre, Benjamin - Garre Hernández, María Soledad - Lubos, Marta - Pícha, Jan - Voburka, Zdeněk - Mitrová, Katarína - Jiráček, Jiří
Combinatorial libraries of bipodal binders of the insulin receptor.
ChemMedChem. Roč. 19, č. 11 (2024), č. článku e202400145. ISSN 1860-7179. E-ISSN 1860-7187
R&D Projects: GA MŠMT(CZ) LX22NPO5104; GA MŠMT(CZ) EF16_019/0000729
Institutional support: RVO:61388963
Keywords : insulin * receptor * library * scaffold * peptide * combinatorial chemistry
OECD category: Medicinal chemistry
Impact factor: 3.4, year: 2022
Method of publishing: Open access
https://doi.org/10.1002/cmdc.202400145

We adopeted a strategy focused on synthesizing extensive combinatorial libraries of bipodal compounds consisting of two distinct peptides linked to a molecular scaffold. These constructs, evaluated in a resin bead-bound format, were designed to assess their binding to the insulin receptor. Despite notable nonspecific binding, our approach successfully generated and tested millions of compounds. Rigorous evaluations via flow cytometry and specific antibodies revealed peptide sequences with specific interactions at either receptor binding Site 1 or 2.
Permanent Link: https://hdl.handle.net/11104/0352016