Inactivation of influenza virus as representative of enveloped RNA viruses on photocatalytically active nanoparticle and nanotubular TiO2 surfaces

0583888 - ÚOCHB 2025 RIV NL eng J - Journal Article
Baudys, M. - Sopha, H. - Hodek, Jan - Rusek, J. - Bartková, H. - Ulrychová, Lucie - Macák, J. M. - Weber, Jan - Krýsa, J.
Inactivation of influenza virus as representative of enveloped RNA viruses on photocatalytically active nanoparticle and nanotubular TiO2 surfaces.
Catalysis Today. Roč. 430, March (2024), č. článku 114511. ISSN 0920-5861. E-ISSN 1873-4308
R&D Projects: GA MŠMT(CZ) LTAIZ19017
Research Infrastructure: CEMNAT III - 90237
Keywords : TiO2 * photocatalysis * nanotubular layer * porosity * virucidal * influenza virus
Impact factor: 5.3, year: 2022
Method of publishing: Limited access
https://doi.org/10.1016/j.cattod.2024.114511

The recent pandemic showed us that there is a strong demand for standardized methods to evaluate the antiviral activity of different materials using enveloped RNA viruses (e.g. SARS-CoV-2, influenza virus). Virucidal activity can be achieved as a result of photoexcitation of a TiO2 photocatalyst under UV illumination. All standardized methods evaluating the virucidal activity of photocatalytic surfaces use bacteriophage Qbeta, a representative of small nonenveloped viruses. This work was thus devoted to the evaluation of the virucidal efficiency of photocatalytically active nanostructured TiO2 surfaces (nanotubular and nanoparticle) to inactivate the influenza virus with particular interest paid to the methodology of virucidal testing and the influence of the surface nanostructure (porosity). Two different TiO2 nanostructures were used in this study, namely nanoparticle and nanotubular structures. A significant decrease in the amount of viral RNA and titre was obtained after rinsing, because the virus was retained on the surface of the nanostructured TiO2 during exposure in the dark. The decrease can be understood as an additional effect of the surface porosity on the TiO2 virucidal activity after UV illumination. However, this fact was taken into account in the calculation of virucidal activity due to UV light. Both nanostructured TiO2 coatings have comparable porosity and thickness, but the photocatalytic activity (to oxidatively degrade aqueous pollutants) is higher for the nanoparticle than for the nanotubular surface. On the other hand, the virucidal activity is much higher for the nanotubular surface. This can be explained by the uniform and open structure of nanotubes resulting in a lower amount of virus being retained on the surface under dark conditions and the high surface area of the nanotubes.
Permanent Link: https://hdl.handle.net/11104/0351876