Sublethal and lethal Cd toxicity in soybean roots specifically affects the metabolome, Cd binding to proteins and cellular distribution of Cd

0583752 - BC 2024 RIV NL eng J - Journal Article
Andresen, Elisa - Flores-Sanchez, Isvett Josefina - Brueckner, D. - Bokhari, Syed Nadeem Hussain - Falkenberg, G. - Küpper, Hendrik
Sublethal and lethal Cd toxicity in soybean roots specifically affects the metabolome, Cd binding to proteins and cellular distribution of Cd.
Journal of Hazardous Materials. Roč. 442, JAN 2023 (2023), č. článku 130062. ISSN 0304-3894. E-ISSN 1873-3336
R&D Projects: GA MŠMT EF15_003/0000336
Institutional support: RVO:60077344
Keywords : Metabolites * Metalloproteomics * Metal stress * Sublethal toxicity
OECD category: Biochemistry and molecular biology
Impact factor: 13.6, year: 2022
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S0304389422018568?via%3Dihub

Soybean (Glycine max (L.) Merr.) plants were exposed to various Cd concentrations from background and low non-toxic (0.5-50 nM) via sublethally toxic (< 550 nM) to highly, ultimately lethally toxic (3 mu M) concentrations. Plants were cultivated hydroponically for 10 weeks until pod development stage of the control plants. The threshold and mechanism of sublethal Cd toxicity was investigated by metabolomics and metalloproteomics (HPLC-ICP-MS) measuring metal binding to proteins in the harvested roots. Spatial distribution of Cd was revealed by mu XRF-CT. Specific binding of Cd to proteins already at 50 nM Cd revealed the likely high-affinity protein binding targets in roots, identified by protein purification from natural abundance. This revealed allantoinase, aquaporins, peroxidases and protein disulfide isomerase as the most likely high-affinity targets of Cd binding. Cd was deposited in cortex cell vacuoles at sublethal and bound to the cell walls of the outer cortex and the vascular bundle at lethal Cd. Cd binding to proteins likely inhibits them, and possibly induces detoxification mechanisms, as verified by metabolomics: allantoic acid and allantoate increased due to sublethal Cd toxicity. Changes of the Cd binding pattern indicated a detoxification strategy at lower Cd, but saturated binding sites at higher Cd concentrations.
Permanent Link: https://hdl.handle.net/11104/0352308