Conservation of triplet-triplet energy transfer photoprotective pathways in fucoxanthin chlorophyll-binding proteins across algal lineages

0583540 - BC 2024 RIV NL eng J - Journal Article
Agostini, Alessandro - Bína, David - Carbonera, D. - Litvín, Radek
Conservation of triplet-triplet energy transfer photoprotective pathways in fucoxanthin chlorophyll-binding proteins across algal lineages.
Biochimica Et Biophysica Acta-Bioenergetics. Roč. 1864, č. 2 (2023), č. článku 148935. ISSN 0005-2728. E-ISSN 1879-2650
R&D Projects: GA ČR(CZ) GA20-01159S; GA MŠMT(CZ) EF18_053/0016982
Institutional support: RVO:60077344
Keywords : light-harvesting complex * emiliania-huxleyi prymnesiophyceae * electron-paramagnetic-resonance * diatom cyclotella-meneghiniana * time-resolved epr * photosynthetic antenna * raman-spectroscopy * Light-harvesting complex * Carotenoid
OECD category: Biophysics
Impact factor: 4.3, year: 2022
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S0005272822004054?via%3Dihub

Detailed information on the photo-generated triplet states of diatom and haptophyte Fucoxanthin Chlorophyllbinding Proteins (FCPs and E-FCPs, respectively) have been obtained from a combined spectroscopic investi-gation involving Transient Absorption and Time-Resolved Electron Paramagnetic Resonance. Pennate diatom Phaeodactylum tricornutum FCP shows identical photoprotective Triplet-Triplet Energy Transfer (TTET) pathways to the previously investigated centric diatom Cyclotella meneghiniana FCP, with the same two chlorophyll a- fucoxanthin pairs that involve the fucoxanthins in sites Fx301 and Fx302 contributing to TTET in both diatom groups. In the case of the haptophyte Emilianina huxleyi E-FCP, only one of the two chlorophyll a-fucoxanthins pairs observed in diatoms, the one involving chlorophyll a409 and Fx301, has been shown to be active in TTET. Furthermore, despite the marked change in the pigment content of E-FCP with growth light intensity, the TTET pathway is not affected. Thus, our comparative investigation of FCPs revealed a photoprotective TTET pathway shared within these classes involving the fucoxanthin in site Fx301, a site exposed to the exterior of the antenna monomer that has no equivalent in Light-Harvesting Complexes from the green lineage.
Permanent Link: https://hdl.handle.net/11104/0352305