Functional metabolomics of the human scalp: a metabolic niche for Staphylococcus epidermidis

0583104 - ÚOCHB 2025 RIV US eng J - Journal Article
Nothias, L-F. - Schmid, Robin - Garlet, A. - Cameron, H. - Leoty-Okombi, S. - Andre-Frei, V. - Fuchs, R. - Dorrestein, P. C. - Ternes, P.
Functional metabolomics of the human scalp: a metabolic niche for Staphylococcus epidermidis.
mSystems. Roč. 9, č. 2 (2024). ISSN 2379-5077. E-ISSN 2379-5077
Institutional support: RVO:61388963
Keywords : metabolomics * skin microbiome * scalp * metabolite annotation * multi-omics integration
Impact factor: 6.4, year: 2022
Method of publishing: Open access
https://doi.org/10.1128/msystems.00356-23

Although metabolomics data acquisition and analysis technologies have become increasingly sophisticated over the past 5-10 years, deciphering a metabolite's function from a description of its structure and its abundance in a given experimental setting is still a major scientific and intellectual challenge. To point out ways to address this "data to knowledge" challenge, we developed a functional metabolomics strategy that combines state-of-the-art data analysis tools and applied it to a human scalp metabolomics data set: skin swabs from healthy volunteers with normal or oily scalp (Sebumeter score 60-120, n = 33, Sebumeter score > 120, n = 41) were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), yielding four metabolomics data sets for reversed phase chromatography (C18) or hydrophilic interaction chromatography (HILIC) separation in electrospray ionization (ESI) + or ionization mode. Following our data analysis strategy, we were able to obtain increasingly comprehensive structural and functional annotations, by applying the Global Natural Product Social Networking (M. Wang, J. J. Carver, V. V. Phelan, L. M. Sanchez, et al., Nat Biotechnol 34:828-837, 2016, https://doi.org/10.1038/nbt.3597), SIRIUS (K. Duhrkop, M. Fleischauer, M. Ludwig, A. A. Aksenov, et al., Nat Methods 16:299-302, 2019, https://doi.org/10.1038/s41592-019-0344-8), and MicrobeMASST (S. ZuffaS, R. Schmid, A. Bauermeister, P. W, P. Gomes, et al., bioRxiv:rs.3.rs-3189768, 2023, https://doi.org/10.21203/rs.3.rs-3189768/v1) tools. We finally combined the metabolomics data with a corresponding metagenomic sequencing data set using MMvec (J. T. Morton, A. A. Aksenov, L. F. Nothias, J. R. Foulds, et. al., Nat Methods 16:1306-1314, 2019, https://doi.org/10.1038/s41592-019-0616-3), gaining insights into the metabolic niche of one of the most prominent microbes on the human skin, Staphylococcus epidermidis.
Permanent Link: https://hdl.handle.net/11104/0351103