Microtubule-associated proteins MAP7 and MAP7D1 promote DNA double-strand break repair in the G1 cell cycle phase

0582795 - ÚOCHB 2024 RIV US eng J - Journal Article
Dullovi, A. - Ozgencil, M. - Rajvee, V. - Tse, W. Y. - Cutillas, P. R. - Martin, S. A. - Hořejší, Zuzana
Microtubule-associated proteins MAP7 and MAP7D1 promote DNA double-strand break repair in the G1 cell cycle phase.
iScience. Roč. 26, č. 3 (2023), č. článku 106107. E-ISSN 2589-0042
Institutional support: RVO:61388963
Keywords : DNA damage * microtubules * microtubule-associated proteins
OECD category: Biochemistry and molecular biology
Impact factor: 5.8, year: 2022
Method of publishing: Open access
https://doi.org/10.1016/j.isci.2023.106107

The DNA-damage response is a complex signaling network that guards genomic integrity. The microtubule cytoskeleton is involved in the repair of DNA doublestrand breaks, however, little is known about which cytoskeleton-related pro-teins are involved in DNA repair and how. Using quantitative proteomics, we discovered that microtubule associated proteins MAP7 and MAP7D1 interact with several DNA repair proteins including DNA double-strand break repair pro-teins RAD50, BRCA1 and 53BP1. We observed that downregulation of MAP7 and MAP7D1 leads to increased phosphorylation of p53 after girradiation. More-over, we determined that the downregulation of MAP7D1 leads to a strong G1 arrest and that the downregulation of MAP7 and MAP7D1 in G1 arrested cells negatively affects DNA repair, recruitment of RAD50 to chromatin and localiza-tion of 53BP1 to the sites of damage. These findings describe for the first time a novel function of MAP7 and MAP7D1 in cell cycle regulation and repair of DNA double-strand breaks.
Permanent Link: https://hdl.handle.net/11104/0350849