EasyClick: an improved system for confocal microscopy of live roots with a user-optimized sample holder

0582673 - ÚEB 2024 RIV DE eng J - Journal Article
Kaduchová, Kateřina - Čmiel, F. - Koláčková, Veronika - Pečinka, Aleš
EasyClick: an improved system for confocal microscopy of live roots with a user-optimized sample holder.
Planta. Roč. 259, č. 1 (2024), č. článku 22. ISSN 0032-0935. E-ISSN 1432-2048
R&D Projects: GA ČR(CZ) GA21-02929S
Institutional support: RVO:61389030
Keywords : Barley * Confocal microscopy * EasyClick * Growth * Hordeum vulgare * Live cell imaging * Plant * Root
OECD category: Cell biology
Impact factor: 4.3, year: 2022
Method of publishing: Open access
https://doi.org/10.1007/s00425-023-04293-y

Preparation and mounting of the samples are key factors for successful live cell microscopy. To acquire biologically relevant data, it is necessary to minimize stress and avoid physical damage to plant tissues during the installation of the sample into the microscope. This is challenging, particularly when the whole plant is mounted as the living sample needs to be properly anchored in the microscopic system to obtain high-quality and high-resolution data. Here, we present a user-optimized sample holder EasyClick for live cell inverted confocal microscopic analysis of plant roots with diameters from 0.3 to 0.7 mm. The EasyClick holder was tested on an inverted confocal microscope using germinating plants of several cereals. Nevertheless, it can be directly used on other types of inverted microscopes from various producers and on different plant species. The EasyClick holder effectively restricts root lateral and vertical movements. This greatly improves the conditions for time-lapse microscopy of the samples of interest.
Permanent Link: https://hdl.handle.net/11104/0350752