Elevated glutathione in researchers exposed to engineered nanoparticles due to potential adaptation to oxidative stress

0582145 - ÚFCH JH 2025 RIV GB eng J - Journal Article
Klusáčková, P. - Lischková, L. - Kolesnikova, V. - Navrátil, Tomáš - Vlčková, Š. - Fenclová, Z. - Schwarz, Jaroslav - Ondráček, Jakub - Ondráčková, Lucie - Koštejn, Martin - Dvořáčková, Š. - Rössnerová, Andrea - Pohanka, M. - Bradna, P. - Ždímal, Vladimír - Pelclová, D.
Elevated glutathione in researchers exposed to engineered nanoparticles due to potential adaptation to oxidative stress.
Nanomedicine. Roč. 19, č. 3 (2024), s. 185-198. ISSN 1743-5889. E-ISSN 1748-6963
Institutional support: RVO:61388955 ; RVO:67985858 ; RVO:68378041
Keywords : adaptation * antioxidant status * inhalation
OECD category: Physical chemistry; Occupational health (UCHP-M)
Impact factor: 5.5, year: 2022
Method of publishing: Open access
https://www.futuremedicine.com/doi/10.2217/nnm-2023-0207

Aim: To find a practical biomonitoring method for researchers exposed to nanoparticles causing oxidative stress. Methods: In a continuation of a study in 2016–2018, biological samples (plasma, urine and exhaled breath condensate [EBC]) were collected in 2019–2020 from 43 researchers (13.8 ± 3.0 years of exposure) and 45 controls. Antioxidant status was assessed using glutathione (GSH) and ferric-reducing antioxidant power, while oxidative stress was measured as thiobarbituric acid reactive substances, all using spectrophotometric methods. Researchers’ personal nanoparticle exposure was monitored. Results: Plasma GSH was elevated in researchers both before and after exposure (p < 0.01), postexposure plasma GSH correlated with nanoparticle exposure, and GSH in EBC increased. Conclusion: The results suggest adaptation to chronic exposure to nanoparticles, as monitored by plasma and EBC GSH.
Permanent Link: https://hdl.handle.net/11104/0350220