A new role of sigma factors in regulation of bacterial transcription

0581866 - MBÚ 2024 RIV CZ eng A - Abstract
Pospíšil, Jiří - Schwarz, Marek - Ziková, Alice - Vítovská, Dragana - Hradilová, Miluše - Kolář, Michal - Křenková, Alena - Hubálek, Martin - Krásný, Libor - Vohradský, Jiří
A new role of sigma factors in regulation of bacterial transcription.
Czech Chem. Soc. Symp. Series. Roč. 21, č. 5 (2023), s. 210-210
[Annual meeting of the National Institute of Virology and Bacteriology (NIVB) /2./. 02.10.2023-05.10.2023, Kutná Hora]
R&D Projects: GA MŠMT(CZ) LX22NPO5103
Institutional support: RVO:61388971 ; RVO:68378050 ; RVO:61388963
Keywords : gene expression * sigma factors * RNA polymerase
OECD category: Microbiology; Biochemistry and molecular biology (UMG-J); Analytical chemistry (UOCHB-X)

Bacterial gene expression is controlled by sigma (σ) factors, that bind to RNA polymerase (RNAP), recognize specific promoter sequences in DNA, and mediate transcription initiation. Bacteria always contain one primary or so called “housekeeping” σ factor. In the gram-positive antibiotic producing bacterium, Streptomyces coelicolor, 65 genes encoding different σ factors are found. HrdB is the main sigma factor in S. coelicolor and is responsible for expression of essential genes. On the other hand, genes that are required in different stresses or environmental conditions are regulated by alternative σ factors. Alternative σ factors are typically about half the size (~30 kDa) of HrdB and lack some of its domains. These factors recognize promoter consensus sequences different from HrdB. They are responsible for transcription of gene subsets with specific functions1,2
Permanent Link: https://hdl.handle.net/11104/0350054