Combining Thermal Desorption with Selected Ion Flow Tube Mass Spectrometry for Analyses of Breath Volatile Organic Compounds.

0581835 - ÚFCH JH 2025 RIV US eng J - Journal Article
Belluomo, I. - Whitlock, S. E. - Myridakis, A. - Parker, A. G. - Converso, V. - Perkins, M. J. - Langford, V. S. - Španěl, Patrik - Hanna, G. B.
Combining Thermal Desorption with Selected Ion Flow Tube Mass Spectrometry for Analyses of Breath Volatile Organic Compounds.
Analytical Chemistry. Roč. 96, č. 4 (2024), s. 1397-1401. ISSN 0003-2700. E-ISSN 1520-6882
Institutional support: RVO:61388955
Keywords : Calibration * Desorption * Ions
OECD category: Physical chemistry
Impact factor: 7.4, year: 2022
Method of publishing: Open access

An instrument integrating thermal desorption (TD) to selected ion flow tube mass spectrometry (SIFT-MS) is presented, and its application to analyze volatile organic compounds (VOCs) in human breath is demonstrated for the first time. The rationale behind this development is the need to analyze breath samples in large-scale multicenter clinical projects involving thousands of patients recruited in different hospitals. Following adapted guidelines for validating analytical techniques, we developed and validated a targeted analytical method for 21 compounds of diverse chemical class, chosen for their clinical and biological relevance. Validation has been carried out by two independent laboratories, using calibration standards and real breath samples from healthy volunteers. The merging of SIFT-MS and TD integrates the rapid analytical capabilities of SIFT-MS with the capacity to collect breath samples across multiple hospitals. Thanks to these features, the novel instrument has the potential to be easily employed in clinical practice.
Permanent Link: https://hdl.handle.net/11104/0350239