Molecular weight and gut microbiota determine the bioavailability of orally administered hyaluronic acid

0581799 - BFÚ 2024 RIV NL eng J - Journal Article
Šimek, M. - Turková, Kristýna - Schwarzer, Martin - Nešporová, K. - Kubala, Lukáš - Hermannová, M. - Foglova, T. - Šafránková, B. - Šindelář, Martin - Šrůtková, Dagmar - Chatzigeorgiou, S. - Novotná, Tereza - Hudcovic, Tomáš - Velebný, V.
Molecular weight and gut microbiota determine the bioavailability of orally administered hyaluronic acid.
Carbohydrate Polymers. Roč. 313, AUG 1 2023 (2023), č. článku 120880. ISSN 0144-8617. E-ISSN 1879-1344
R&D Projects: GA ČR(CZ) GM21-19640M
Institutional support: RVO:68081707 ; RVO:61388971
Keywords : chondroitin sulfate * absorption * tissue * cells * rats * Microbiota * Hyaluronan * Metabolism * Bioavailability * Digestion * Oligosaccharides
OECD category: Polymer science; Polymer science (MBU-M)
Impact factor: 11.2, year: 2022
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0144861723003454?via%3Dihub

The ability of hyaluronan as a dietary supplement to increase skin moisture and relieve knee pain has been demonstrated in several clinical studies. To understand the mechanism of action, determining hyaluronan's bioavailability and in vivo fate is crucial. Here, we used 13C-hyaluronan combined with LC-MS analysis to compare the absorption and metabolism of oral hyaluronan in germ-free and conventional wild-type mice. The presence of Bacteroides spp. in the gut was crucial for hyaluronan absorption. Specific microorganisms cleave hyaluronan into unsaturated oligosaccharides (<3 kDa) which are partially absorbed through the intestinal wall. The remaining hyaluronan fragments are metabolized into short-chain fatty acids, which are only metabolites available to the host. The poor bioavailability (similar to 0.2 %) of oral hyaluronan indicates that the mechanism of action is the result of the systematic regulatory function of hyaluronan or its metabolites rather than the direct effects of hyaluronan at distal sites of action (skin, joints).
Permanent Link: https://hdl.handle.net/11104/0349956