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Karyotypes of water frogs from the Pelophylax esculentus complex: results of cross-species chromosomal painting

  1. 1.
    0581729 - ÚŽFG 2024 RIV DE eng J - Journal Article
    Dedukh, Dmitrij - Maslova, A. - Al-Rikabi, A. B. H. - Padutsch, N. - Liehr, T. - Krasikova, A.
    Karyotypes of water frogs from the Pelophylax esculentus complex: results of cross-species chromosomal painting.
    Chromosoma. Roč. 132, č. 4 (2023), s. 329-342. ISSN 0009-5915. E-ISSN 1432-0886
    R&D Projects: GA ČR(CZ) GF23-07028K
    Institutional support: RVO:67985904
    Keywords : cytogenetics * anura * Cytogenetics * Chromosome evolution * Clonal hybrids * Pelophylax esculentus complex * Chromosomal painting * Fluorescence in situ hybridization
    OECD category: Zoology
    Impact factor: 1.6, year: 2022
    Method of publishing: Limited access
    https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=99503336992

    Amphibian species have the largest genome size enriched with repetitive sequences and relatively similar karyotypes. Moreover, many amphibian species frequently hybridize causing nuclear and mitochondrial genome introgressions. In addition, hybridization in some amphibian species may lead to clonality and polyploidization. All such events were found in water frogs from the genus Pelophylax. Among the species within the genus Pelophylax, P. esculentus complex is the most widely distributed and well-studied. This complex includes two parental species, P. ridibundus and P. lessonae, and their hybrids, P. esculentus, reproducing hemiclonally. Parental species and their hybrids have similar but slightly polymorphic karyotypes, so their precise identification is still required. Here, we have developed a complete set of 13 chromosome painting probes for two parental species allowing the precise identification of all chromosomes. Applying chromosomal painting, we identified homologous chromosomes in both parental species and orthologous chromosomes in their diploid hemiclonal hybrids. Comparative painting did not reveal interchromosomal exchanges between the studied water frog species and their hybrids. Using cross-specific chromosome painting, we detected unequal distribution of the signals along chromosomes suggesting the presence of species-specific tandem repeats. Application of chromosomal paints to the karyotypes of hybrids revealed differences in the intensity of staining for P. ridibundus and P. lessonae chromosomes. Thus, both parental genomes have a divergence in unique sequences. Obtained chromosome probes may serve as a powerful tool to unravel chromosomal evolution in phylogenetically related species, identify individual chromosomes in different cell types, and investigate the elimination of chromosomes in hybrid water frogs.
    Permanent Link: https://hdl.handle.net/11104/0350026

     
     
Number of the records: 1  

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