Dermomyotome-derived endothelial cells migrate to the dorsal aorta to support hematopoietic stem cell emergence

0581622 - ÚMG 2024 RIV GB eng J - Journal Article
Sahai-Hernandez, P. - Pouget, C. - Eyal, S. - Svoboda, Ondřej - Chacon, J. - Grimm, L. - Gjøen, T. - Traver, D.
Dermomyotome-derived endothelial cells migrate to the dorsal aorta to support hematopoietic stem cell emergence.
eLife. Roč. 12, Sep (2023), č. článku e58300. ISSN 2050-084X. E-ISSN 2050-084X
Institutional support: RVO:68378050
Keywords : adult bloodforming system * zebrafish * dorsal aorta * Development
OECD category: Cell biology
Impact factor: 7.7, year: 2022
Method of publishing: Open access
https://elifesciences.org/articles/58300

Development of the dorsal aorta is a key step in the establishment of the adult blood-forming system, since hematopoietic stem and progenitor cells (HSPCs) arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here, we profile the transcriptome of the earliest detectable endothelial cells (ECs) during zebrafish embryogenesis to demonstrate that tissue-specific EC programs initiate much earlier than previously appreciated, by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite-derived endothelial cells (SDECs) that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish, where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Although SDECs lack hematopoietic potential, they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus, at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs, hemogenic ECs, and SDECs. Taken together, our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta.
Permanent Link: https://hdl.handle.net/11104/0349734