Commissioning the digital mass-filter/ion-trap module for the MS SPIDOC prototype

0581604 - MBÚ 2024 RIV US eng J - Journal Article
Simke, F. - Fischer, P. - Damjanović, T. - Kádek, Alan - Kierspel, T. - Lorenzen, K. - Uetrecht, C. - Schweikhard, L.
Commissioning the digital mass-filter/ion-trap module for the MS SPIDOC prototype.
Journal of Instrumentation. Roč. 18, č. 10 (2023), č. článku P10021. ISSN 1748-0221. E-ISSN 1748-0221
Institutional support: RVO:61388971
Keywords : Instrumentation for FEL * Instrumentation for radioactive beams (fragmentation devices, fragment and isotope, separators incl. ISOL, isobar separators, ion and atom traps, weak-beam diagnostics, radioactive-beam ion sources) * Mass spectrometers
OECD category: Analytical chemistry
Impact factor: 1.3, year: 2022
Method of publishing: Open access
https://iopscience.iop.org/article/10.1088/1748-0221/18/10/P10021

The combination of a linear quadrupole ion-filter and linear Paul trap operated with a rectangular guiding field for the filtering and accumulation of ions within the Mass Spectrometry for Single Particle Imaging of Dipole Oriented protein Complexes (MS SPIDOC) prototype [T. Kierspel et al., Anal. Bioanal. Chem., published online] is characterized. Using cationic caesium-iodide clusters, the ion-separation performance, ion accumulation, cooling, and ejection via in-trap pin electrodes is evaluated. Furthermore, proof-of-principle measurements are performed with 64 kDa multiply-charged non-covalent protein complexes of human hemoglobin and 804 kDa non-covalent complex of GroEL, to demonstrate that the module meets the criteria to handle high-mass ions which are the main objective of the MS SPIDOC project. The setup's performance is found to be in line with previous results from ion-trajectory simulations [F. Simke et al., Int. J. Mass Spectrom. 473 (2022) 116779].
Permanent Link: https://hdl.handle.net/11104/0349707