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Plant Cytogenetics and Cytogenomics, Methods in Molecular Biology
- 1.0580633 - BÚ 2024 RIV US eng M - Monography Chapter
Loureiro, J. - Čertner, Martin - Lučanová, Magdalena - Sliwinska, E. - Kolář, Filip - Doležel, Jaroslav - Garcia, S. - Castro, S. - Galbraith, D. W.
The Use of Flow Cytometry for Estimating Genome Sizes and DNA Ploidy Levels in Plants.
Plant Cytogenetics and Cytogenomics, Methods in Molecular Biology. New York: Humana, 2023 - (Heitkam, T.; Garcia, S.), s. 25-64. ISBN 978-1-0716-3225-3
Institutional support: RVO:67985939 ; RVO:61389030
Keywords : DNA ploidy level * flow cytometry * genome size
OECD category: Plant sciences, botany
https://doi.org/10.1007/978-1-0716-3226-0
In this book chapter we aim to provide general guidelines and detailed protocols for the measurement of nuclear DNA contents in plants using flow cytometry. Besides providing a step-by-step guide for estimating genome sizes and DNA ploidy levels from fresh tissues, special attention is given to the singularities of using seeds and desiccated tissues for such purposes. Methodological aspects regarding field sampling, transport and storage of plant material are also given in detail.
Permanent Link: https://hdl.handle.net/11104/0349405
Number of the records: 1