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σE of Streptomyces coelicolor can function both as a direct activator or repressor of transcription
- 1.0580571 - MBÚ 2025 RIV GB eng J - Journal Article
Pospíšil, Jiří - Schwarz, Marek - Ziková, Alice - Vítovská, Dragana - Hradilová, Miluše - Kolář, Michal - Křenková, Alena - Hubálek, Martin - Krásný, Libor - Vohradský, Jiří
σE of Streptomyces coelicolor can function both as a direct activator or repressor of transcription.
Communications Biology. Roč. 7, January 6 (2024), č. článku 46. E-ISSN 2399-3642
R&D Projects: GA ČR(CZ) GF22-06342K; GA MŠMT(CZ) LM2023055; GA MŠMT(CZ) LX22NPO5103
Research Infrastructure: ELIXIR CZ III - 90255
Institutional support: RVO:61388971 ; RVO:68378050 ; RVO:61388963
Keywords : σ factors * proteins * Streptomyces coelicolor * gene expression
OECD category: Microbiology; Biochemistry and molecular biology (UMG-J)
Impact factor: 5.9, year: 2022
Method of publishing: Open access
https://www.nature.com/articles/s42003-023-05716-y
σ factors are considered as positive regulators of gene expression. Here we reveal the opposite, inhibitory role of these proteins. We used a combination of molecular biology methods and computational modeling to analyze the regulatory activity of the extracytoplasmic σE factor from Streptomyces coelicolor. The direct activator/repressor function of σE was then explored by experimental analysis of selected promoter regions in vivo. Additionally, the σE interactome was defined. Taken together, the results characterize σE, its regulation, regulon, and suggest its direct inhibitory function (as a repressor) in gene expression, a phenomenon that may be common also to other σ factors and organisms.
Permanent Link: https://hdl.handle.net/11104/0349344
File Download Size Commentary Version Access 2024_Pospisil_Communications Biology.pdf 1 2.8 MB Publisher’s postprint open-access
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