Electrochemistry in sensing of molecular interactions of proteins and their behavior in an electric field

0580464 - ÚFE 2024 RIV AT eng J - Journal Article
Vacek, J. - Zatloukalová, M. - Dorčák, V. - Cifra, Michal - Futera, Z. - Ostatná, Veronika
Electrochemistry in sensing of molecular interactions of proteins and their behavior in an electric field.
Microchimica Acta. Roč. 190, č. 11 (2023), č. článku 442. ISSN 0026-3672. E-ISSN 1436-5073
R&D Projects: GA ČR(CZ) GX20-06873X; GA ČR(CZ) GA22-26590S
Institutional support: RVO:67985882 ; RVO:68081707
Keywords : Electrode * Microdevice * Peptide * Protein * Sensor
OECD category: Electrical and electronic engineering; Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) (BFU-R)
Impact factor: 5.7, year: 2022
Method of publishing: Open access

Electrochemical methods can be used not only for the sensitive analysis of proteins but also for deeper research into their structure, transport functions (transfer of electrons and protons), and sensing their interactions with soft and solid surfaces. Last but not least, electrochemical tools are useful for investigating the effect of an electric field on protein structure, the direct application of electrochemical methods for controlling protein function, or the micromanipulation of supramolecular protein structures. There are many experimental arrangements (modalities), from the classic configuration that works with an electrochemical cell to miniaturized electrochemical sensors and microchip platforms. The support of computational chemistry methods which appropriately complement the interpretation framework of experimental results is also important. This text describes recent directions in electrochemical methods for the determination of proteins and briefly summarizes available methodologies for the selective labeling of proteins using redox-active probes. Attention is also paid to the theoretical aspects of electron transport and the effect of an external electric field on the structure of selected proteins. Instead of providing a comprehensive overview, we aim to highlight areas of interest that have not been summarized recently, but, at the same time, represent current trends in the field
Permanent Link: https://hdl.handle.net/11104/0349230