Microbial Environmental Genomics (MEG)

0580396 - MBÚ 2024 RIV US eng M - Monography Chapter
Štursová, Martina - López-Mondejár, Rubén - Baldrian, Petr
Investigating the Bacterial and Fungal Communities Involved in Dead Biomass Degradation in Forest Soils.
Microbial Environmental Genomics (MEG). New York: Humana, 2023 - (Martin, F.; Uroz, S.), s. 157-168. Methods in Molecular Biology. ISBN 978-1-0716-2870-6
R&D Projects: GA ČR(CZ) GA20-14961S; GA ČR(CZ) GA22-30769S
Institutional support: RVO:61388971
Keywords : Stable isotope probing * Microbial communities * Soil ecology * Organic matter decomposition * Metagenomics * metatranscriptomics
OECD category: Microbiology

Stable isotope probing (SIP) provides the opportunity to label decomposer microorganisms that build their biomass on a specific substrate. In combination with high-throughput sequencing, SIP allows for the identification of microbial community members involved in a particular decomposition process. Further information can be gained (in SIP experiments) through gene-targeted metagenomics and metatranscriptomics, opening the possibility to describe the pool of genes catalyzing specific decomposition reactions in situ and to identify the diversity of genes that are expressed. When combined with gene descriptions of fungal and/or bacterial isolates from the same environment, specific biochemical reactions involved in decomposition can be linked to individual microbial taxa. Here, we describe the use of these methods to explore the decomposer community of fungi and bacteria in forest litter and soil.
Permanent Link: https://hdl.handle.net/11104/0349169