Microfluidic device for enhancement and analysis of osteoblast differentiation in three-dimensional cell cultures

0580038 - ÚIACH 2024 RIV GB eng J - Journal Article
Killinger, Michael - Kratochvílová, Adéla - Ingeborg Reihs, E. - Matalová, Eva - Klepárník, Karel - Rothbauer, M.
Microfluidic device for enhancement and analysis of osteoblast differentiation in three-dimensional cell cultures.
Journal of Biological Engineering. Roč. 17, č. 1 (2023), č. článku 77. ISSN 1754-1611. E-ISSN 1754-1611
R&D Projects: GA ČR(CZ) GA20-00726S
Institutional support: RVO:68081715 ; RVO:67985904
Keywords : bone on a chip * microwells * micropillars
OECD category: Analytical chemistry; Cell biology (UZFG-Y)
Impact factor: 5.6, year: 2022
Method of publishing: Open access
https://doi.org/10.1186/s13036-023-00395-z

In this study, we consequently developed, optimized and characterized an advanced perfused microfluidic platform to improve the reliability of 3D bone cell cultivation and to enhance aspects of bone tissue maturation in vitro. Moreover, biomechanical stimulation generated by fluid flow inside the arrayed chamber, was used to mimic a more dynamic cell environment emulating a highly vascularized bone we expected to improve the osteogenic 3D microenvironment in the developed multifunctional spheroid-array platform.
Permanent Link: https://hdl.handle.net/11104/0348820