Conjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency.

0579986 - MBÚ 2024 RIV US eng J - Journal Article
Pourali, Parastoo - Dzmitruk, Volha - Benada, Oldřich - Svoboda, Milan - Benson, Veronika
Conjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency.
Scientific Reports. Roč. 13, September 6 (2023), s. 14669. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA MŠMT(CZ) EH22_010/0002357; GA MŠMT(CZ) LM2018127
Research Infrastructure: CIISB II - 90127
Institutional support: RVO:61388971 ; RVO:86652036 ; RVO:68081715
Keywords : gold nanoparticles * capping agent * biosynthesis * nucleic acid transfection * drug carriers
OECD category: Microbiology
Impact factor: 4.6, year: 2022
Method of publishing: Open access
https://www.nature.com/articles/s41598-023-41567-7

In this study, gold nanoparticles produced by eukaryotic cell waste (AuNP), were analyzed as a transfection tool. AuNP were produced by Fusarium oxysporum and analyzed by spectrophotometry, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS). Fourier transform infrared spectroscopy (FTIR) and dynamic light scattering (DLS) were used before and after conjugation with different nucleic acid (NA) types. Graphite furnace atomic absorption spectroscopy (GF-AAS) was used to determine the AuNP concentration. Conjugation was detected by electrophoresis. Confocal microscopy and quantitative real-time PCR (qPCR) were used to assess transfection. TEM, SEM, and EDS showed 25nm AuNP with round shape. The amount of AuNP was 3.75±0.2g/L and FTIR proved conjugation of all NA types to AuNP. All the samples had a negative charge of36 to46mV. Confocal microscopy confirmed internalization of the ssRNA-AuNP into eukaryotic cells and qPCR confirmed release and activity of carried RNA.
Permanent Link: https://hdl.handle.net/11104/0348880