Multiple Sgip1 splice variants inhibit cannabinoid receptor 1 internalization

0579881 - ÚMG 2025 RIV NL eng J - Journal Article
Durydivka, Oleh - Gazdarica, Matej - Večerková, Kateřina - Radenkovic, Silvia - Blahoš, Jaroslav
Multiple Sgip1 splice variants inhibit cannabinoid receptor 1 internalization.
Gene. Roč. 892, Jan (2024), č. článku 147851. ISSN 0378-1119. E-ISSN 1879-0038
R&D Projects: GA ČR GA19-24172S; GA ČR GA21-02371S; GA MŠMT(CZ) LM2023050; GA MŠMT LM2023036; GA MŠMT EF18_046/0015861
Institutional support: RVO:68378050
Keywords : amino-acid-composition * protein-phosphorylation * platform * association * enrichment * domain * Alternative splicing * Cannabinoid receptor * Clathrin-mediated endocytosis * Isoform * Molecular cloning * RNA
OECD category: Cell biology
Impact factor: 3.5, year: 2022
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S0378111923006923?via%3Dihub

Alternative splicing can often result in the expression of distinct protein isoforms from a single gene, with specific composition and properties. SH3-containing GRB2-like protein 3-interacting protein 1 (Sgip1) is a brain-enriched protein that regulates clathrin-mediated endocytosis and interferes with the internalization of cannabinoid receptor 1. Several research groups have studied the physiological importance of Sgip1, and four Sgip1 protein isoforms have been described to date, while the NCBI Gene database predicts the expression of 20 splice variants from the Sgip1 gene in mice. In this work, we cloned 15 Sgip1 splice variants from the mouse brain, including 11 novel splice variants. The cloned splice variants differed in exon composition within two Sgip1 regions: the membrane phospholipid-binding domain and the proline-rich region. All the Sgip1 splice isoforms had similar stability and comparable ability to inhibit the internalization of cannabinoid receptor 1. None of the isoforms influenced the internalization of the mu-opioid receptor. We confirm the expression of Sgip1 splice variants described in previous studies or predicted in silico. Our data provide a basis for further studies exploring the significance of Sgip1 splicing, and we suggest a new classification of Sgip1 splice variants to unify their nomenclature.
Permanent Link: https://hdl.handle.net/11104/0348669