Number of the records: 1
AAK1-like: A putative pseudokinase with potential roles in cargo uptake in bloodstream form iTrypanosoma brucei/i parasites
- 1.0579861 - BC 2024 RIV US eng J - Journal Article
Black, J. - Klinger, C.M. - Lemgruber, L. - Dacks, Joel Bryan - Mottram, J.C. - McCulloch, R.
AAK1-like: A putative pseudokinase with potential roles in cargo uptake in bloodstream form iTrypanosoma brucei/i parasites.
Journal of Eukaryotic Microbiology. Roč. 70, DEC (2023), č. článku e12994. ISSN 1066-5234. E-ISSN 1550-7408
Institutional support: RVO:60077344
Keywords : clathrin-mediated endocytosis * flagellar pocket * structural explanation * phylogenetic analysis * protein * surface * ap-2 * identification * recognition * subunit * aak1 * African trypanosomes * AP-2 complex * endocytosis
OECD category: Microbiology
Impact factor: 2.2, year: 2022
Method of publishing: Open access
https://onlinelibrary.wiley.com/doi/10.1111/jeu.12994
Selection and internalization of cargo via clathrin-mediated endocytosis requires adaptor protein complexes. One complex, AP-2, acts during cargo selection at the plasma membrane. African trypanosomes lack all components of the AP-2 complex, except for a recently identified orthologue of the AP-2-associated protein kinase 1, AAK1. In characterized eukaryotes, AAK1 phosphorylates the mu 2 subunit of the AP-2 complex to enhance cargo recognition and uptake into clathrin-coated vesicles. Here, we show that kinetoplastids encode not one, but two AAK1 orthologues: one (AAK1L2) is absent from salivarian trypanosomes, while the other (AAK1L1) lacks important kinase-specific residues in a range of trypanosomes. These AAK1L1 and AAK1L2 novelties reinforce suggestions of functional divergence in endocytic uptake within salivarian trypanosomes. Despite this, we show that AAK1L1 null mutant Trypanosoma brucei, while viable, display slowed proliferation, morphological abnormalities including swelling of the flagellar pocket, and altered cargo uptake. In summary, our data suggest an unconventional role for a putative pseudokinase during endocytosis and/or vesicular trafficking in T. brucei, independent of AP-2.
Permanent Link: https://hdl.handle.net/11104/0348657
Number of the records: 1