Oral Microbiota Composition and Antimicrobial Antibody Response in Patients with Recurrent Aphthous Stomatitis

0579590 - ÚEM 2024 RIV CH eng J - Journal Article
Stehlíková, Z. - Tláskal, V. - Galanová, N. - Roubalová, L. - Kreisinger, J. - Dvořák, J. - Procházková, P. - Kostovčíková, K. - Bártová, J. - Libanská, M. - Čermáková, R. - Schierová, D. - Fassmann, A. - Linhartová, P. B. - Coufal, Š. - Kverka, Miloslav - Izakovicová-Hollá, L. - Petanová, J. - Tlaskalová-Hogenová, H. - Jirásková Zákostelská, Z.
Oral Microbiota Composition and Antimicrobial Antibody Response in Patients with Recurrent Aphthous Stomatitis.
Microorganisms. Roč. 7, č. 12 (2019), č. článku 636. E-ISSN 2076-2607
Institutional support: RVO:68378041
Keywords : microbiome * oral mucosa * Aphthous Stomatitis
OECD category: Microbiology
Impact factor: 4.152, year: 2019
Method of publishing: Open access
https://www.mdpi.com/2076-2607/7/12/636

Recurrent aphthous stomatitis (RAS) is the most common disease of the oral mucosa, and it has been recently associated with bacterial and fungal dysbiosis. To study this link further, we investigated microbial shifts during RAS manifestation at an ulcer site, in its surroundings, and at an unaffected site, compared with healed mucosa in RAS patients and healthy controls. We sampled microbes from five distinct sites in the oral cavity. The one site with the most pronounced differences in microbial alpha and beta diversity between RAS patients and healthy controls was the lower labial mucosa. Detailed analysis of this particular oral site revealed strict association of the genus Selenomonas with healed mucosa of RAS patients, whereas the class Clostridia and genera Lachnoanaerobaculum, Cardiobacterium, Leptotrichia, and Fusobacterium were associated with the presence of an active ulcer. Furthermore, active ulcers were dominated by Malassezia, which were negatively correlated with Streptococcus and Haemophilus and positively correlated with Porphyromonas species. In addition, RAS patients showed increased serum levels of IgG against Mogibacterium timidum compared with healthy controls. Our study demonstrates that the composition of bacteria and fungi colonizing healthy oral mucosa is changed in active RAS ulcers, and that this alteration persists to some extent even after the ulcer is healed.
Permanent Link: https://hdl.handle.net/11104/0348403