Hydride generation atomic fluorescence spectrometry - searching for minimum detection limits

0579497 - ÚIACH 2024 BR eng A - Abstract
Dědina, Jiří
Hydride generation atomic fluorescence spectrometry - searching for minimum detection limits.
II Workshop on Bioanalytical Chemistry. Pelotas: University of Pelotas (UFPel), 2023. s. 1-2.
[Workshop on Bioanalytical Chemistry /2./. 02.12.2023, Pelotas]
Institutional support: RVO:68081715
Keywords : atomic fluorescence spectrometry (AFS) * electrodeless discharge lamp (EDL) * volatile species * hydride generation
OECD category: Analytical chemistry

Atomic fluorescence spectrometry (AFS) coupled to generation of volatile species has the potential to reach very low limits of detection (LOD). Namely the non- dispersive arrangement of the AFS apparatus provides an attractive alternative even to mass spectrometric techniques because of low purchase and operating costs. The potential of AFS to reach very low LODs can generally be fully achieved only when using very mild atomization conditions which are typically compatible with the atomization of volatile species. This is the reason why AFS is currently associated prevalently with volatile species. The simple and convenient non-dispersive AFS apparatus employs an interference filter for the selection of the detection wavelength interval placed in front of a detector, typically a photomultiplier. The other two essential components of a non-dispersive atomic fluorescence spectrometer include an atomizer and a radiation source (atomic lamp). Since the introduction of non-dispersive AFS as an analytical method mainly two types of atomic lamps have been used: electrodeless discharge lamp (EDL) or boosted hollow cathode lamp. EDLs are said to provide higher radiation intensities, however, the range of elements for which the EDLs are manufactured is limited to volatile elements. Boosted hollow cathode lamps are widely applied in the current commercial AFS instruments whereas EDLs are usually used in experimental laboratory setups of AFS. The relevant instrumental settings for a non- dispersive AFS apparatus are namely: type of atomic lamp; atomic lamp feeding power/current and its modulation pattern; optical path parameters such as focusing of (i) radiation from the atomic lamp to the atomizer and (ii) fluorescence radiation to the photomultiplier; choice of a suitable interference filter; and selection of the optimum fluorescence line. In principle, the intensity of the fluorescence radiation is proportional to the radiation source intensity.
Permanent Link: https://hdl.handle.net/11104/0348335