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Hematopoietic stem cells undergo a lymphoid to myeloid switch in early stages of emergency granulopoiesis
- 1.0579429 - ÚMG 2024 RIV US eng J - Journal Article
Vaníčková, Karolína - Miloševič, Mirko - Ribeiro Bas, Irina - Burocziová, Monika - Yokota, A. - Daněk, Petr - Grušanovič, Srdjan - Chilinski, M. - Plewczynski, D. - Rohlena, Jakub - Hirai, H. - Rohlenová, Kateřina - Alberich-Jorda, Meritxell
Hematopoietic stem cells undergo a lymphoid to myeloid switch in early stages of emergency granulopoiesis.
EMBO Journal. Roč. 42, č. 23 (2023), č. článku e113527. ISSN 0261-4189. E-ISSN 1460-2075
R&D Projects: GA MŠMT LX22NPO5102; GA ČR GA22-18300S
EU Projects: European Commission(XE) 860002 - ENHPATHY
Grant - others:EMBO(DE) 5068-2022
Program: Installation Grant
Institutional support: RVO:68378050 ; RVO:86652036
Keywords : protein-c receptor * c/ebp-beta * progenitor cells * self-renewal * steady-state * gene * differentiation * signals * driven * cd201 * cd201 * emergency granulopoiesis * lymphoid-biased HSC * myeloid-biased HSC
OECD category: Cell biology; Cell biology (BTO-N)
Impact factor: 11.4, year: 2022
Method of publishing: Open access
https://www.embopress.org/doi/epdf/10.15252/embj.2023113527?src=getftr
Emergency granulopoiesis is the enhanced and accelerated production of granulocytes that occurs during acute infection. The contribution of hematopoietic stem cells (HSCs) to this process was reported, however, how HSCs participate in emergency granulopoiesis remains elusive. Here, using a mouse model of emergency granulopoiesis we observe transcriptional changes in HSCs as early as 4 h after lipopolysaccharide (LPS) administration. We observe that the HSC identity is changed towards a myeloid-biased HSC and show that CD201 is enriched in lymphoid-biased HSCs. While CD201 expression under steady-state conditions reveals a lymphoid bias, under emergency granulopoiesis loss of CD201 marks the lymphoid-to-myeloid transcriptional switch. Mechanistically, we determine that lymphoid-biased CD201+ HSCs act as a first response during emergency granulopoiesis due to direct sensing of LPS by TLR4 and downstream activation of NF-kappa Beta signaling. The myeloid-biased CD201- HSC population responds indirectly during an acute infection by sensing G-CSF, increasing STAT3 phosphorylation, and upregulating LAP/LAP* C/EBP beta isoforms. In conclusion, HSC subpopulations support early phases of emergency granulopoiesis due to their transcriptional rewiring from a lymphoid-biased to myeloid-biased population and thus establishing alternative paths to supply elevated numbers of granulocytes.
Permanent Link: https://hdl.handle.net/11104/0348238
File Download Size Commentary Version Access Vanickova-EMBO Journal-2023.pdf 3 3.1 MB Publisher’s postprint open-access
Number of the records: 1