Rapid identification of pathogens in blood serum via Raman tweezers in combination with advanced processing methods

0579192 - ÚPT 2024 RIV US eng J - Journal Article
Vaculík, Ondřej - Bernatová, Silvie - Rebrošová, K. - Samek, Ota - Šilhan, Lukáš - Růžička, F. - Šerý, Mojmír - Šiler, Martin - Ježek, Jan - Zemánek, Pavel
Rapid identification of pathogens in blood serum via Raman tweezers in combination with advanced processing methods.
Biomedical Optics Express. Roč. 14, č. 12 (2023), s. 6410-6421. ISSN 2156-7085
R&D Projects: GA MPO(CZ) FV40455; GA TA ČR(CZ) FW06010453; GA MZd(CZ) NU21-05-00341
Institutional support: RVO:68081731
Keywords : Raman tweezers * blood serum * Raman spectroscopy * bloodstream infections * diagnostics * sepsis
OECD category: Optics (including laser optics and quantum optics)
Impact factor: 3.4, year: 2022
Method of publishing: Open access
https://opg.optica.org/boe/fulltext.cfm?uri=boe-14-12-6410&id=542842

Pathogenic microbes contribute to several major global diseases that kill millions of people every year. Bloodstream infections caused by these microbes are associated with high morbidity and mortality rates, which are among the most common causes of hospitalizations. The search for the “Holy Grail” in clinical diagnostic microbiology, a reliable, accurate, low cost, real-time, and easy-to-use diagnostic method, is one of the essential issues in clinical practice. These very critical conditions can be met by Raman tweezers in combination with advanced analysis methods. Here, we present a proof-of-concept study based on Raman tweezers combined with spectral mixture analysis that allows for the identification of microbial strains directly from human blood serum without user intervention, thus eliminating the influence of a data analyst.
Permanent Link: https://hdl.handle.net/11104/0348043