Approach to map nanotopography of cell surface receptors

0579083 - ÚMG 2024 RIV GB eng J - Journal Article
Franke, Ch. - Chum, T. - Kvíčalová, Z. - Glatzová, Daniela - Gentsch, G. J. - Rodríguez, A. - Helmerich, D. A. - Herdly, L. - Mavila, H. - Frank, O. - Brdička, Tomáš - van de Linde, S. - Cebecauer, M.
Approach to map nanotopography of cell surface receptors.
Communications Biology. Roč. 5, č. 1 (2022), č. článku 218. E-ISSN 2399-3642
R&D Projects: GA MŠMT(CZ) EF16_013/0001775
Institutional support: RVO:68378050
Keywords : electron-microscopy * localization * fluorescence * molecules * adhesion * microclusters * microvilli * palmitoylation * artifacts * epitopes
OECD category: Immunology
Impact factor: 5.9, year: 2022
Method of publishing: Open access
https://www.nature.com/articles/s42003-022-03152-y

Cells communicate with their environment via surface receptors, but nanoscopic receptor organization with respect to complex cell surface morphology remains unclear. This is mainly due to a lack of accessible, robust and high-resolution methods. Here, we present an approach for mapping the topography of receptors at the cell surface with nanometer precision. The method involves coating glass coverslips with glycine, which preserves the fine membrane morphology while allowing immobilized cells to be positioned close to the optical surface. We developed an advanced and simplified algorithm for the analysis of single-molecule localization data acquired in a biplane detection scheme. These advancements enable direct and quantitative mapping of protein distribution on ruffled plasma membranes with near isotropic 3D nanometer resolution. As demonstrated successfully for CD4 and CD45 receptors, the described workflow is a straightforward quantitative technique to study molecules and their interactions at the complex surface nanomorphology of differentiated metazoan cells.
Permanent Link: https://hdl.handle.net/11104/0348325