Tetraploidy in the Boettger's dwarf clawed frog (Pipidae: Hymenochirus boettgeri) from the Congo indicates non-conspecificity with the captive population

0578815 - ÚBO 2025 RIV US eng J - Journal Article
Gvoždík, Václav - Knytl, M. - Zassi-Boulou, A.-G. - Fornaini, N. R. - Bergelová, B.
Tetraploidy in the Boettger's dwarf clawed frog (Pipidae: Hymenochirus boettgeri) from the Congo indicates non-conspecificity with the captive population.
Zoological Journal of the Linnean Society. Roč. 200, č. 4 (2024), s. 1034-1047. ISSN 0024-4082. E-ISSN 1096-3642
Institutional support: RVO:68081766
Keywords : amphibia * Anura * chromosome * cytogenetics * fish * karyotype * polyploidy * taxonomy
OECD category: Zoology
Impact factor: 2.8, year: 2022
Method of publishing: Open access
https://academic.oup.com/zoolinnean/advance-article/doi/10.1093/zoolinnean/zlad119/7321480?login=true

Cytogenetics can be used as a tool to study the evolution of polyploidy and taxonomy. Here we focus on aquatic African pipids, dwarf clawed frogs (Hymenochirus). Our study reveals that dwarf clawed frogs, present for decades in captivity, are best referred to as Hymenochirus sp. instead of the commonly used name 'H. boettgeri' or sometimes 'H. curtipes'. We present the first karyotype from a morphologically identified specimen of H. boettgeri with a known locality in the north-western Congo, which is tetraploid with 2n = 36. The captive Hymenochirus species has been found diploid in previous studies with different reported chromosome numbers, here we reveal 2n = 20A + 1B chromosomes. Our findings suggest that the tetraploid H. boettgeri karyotype evolved through fusion of two biarmed chromosomes and subsequent allotetraploidization, and is functionally diploid, similar to the origin of tetraploid clawed frogs in the subgenus Xenopus. We observed the stable presence of a single B chromosome in both sexes of our individuals from the captive population of Hymenochirus sp. However, additional investigation is necessary to clarify whether there is variation in the number of A and B chromosomes among populations, individuals, and/or tissues. Further research is also needed to understand the evolution and taxonomy of the genus Hymenochirus.
Permanent Link: https://hdl.handle.net/11104/0347712