Chlorophyll biosynthesis under the control of arginine metabolism.

0578358 - MBÚ 2024 RIV US eng J - Journal Article
Kiss, Eva - Talbot, Jana - Adams, N. B. P. - Opekar, Stanislav - Moos, Martin - Pilný, Jan - Kvasov, T. - Schneider, E. - Koník, Peter - Šimek, Petr - Sobotka, Roman
Chlorophyll biosynthesis under the control of arginine metabolism.
Cell Reports. Roč. 42, č. 11 (2023), s. 113265. ISSN 2211-1247. E-ISSN 2211-1247
EU Projects: European Commission(CZ) 854126 - PhotoRedesign
Institutional support: RVO:61388971 ; RVO:60077344
Keywords : arginine metabolism * bilins * chlorophyll * CP: Plants * genome-uncoupled-4 * nitrogen homeostasis * Synechocystis * tetrapyrrole biosynthesis
OECD category: Microbiology; Biochemistry and molecular biology (BC-A)
Impact factor: 8.8, year: 2022
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S2211124723012779

In natural environments, photosynthetic organisms adjust their metabolism to cope with the fluctuating availability of combined nitrogen sources, a growth-limiting factor. For acclimation, the dynamic degradation/synthesisof tetrapyrrolic pigments, as well as of the amino acid arginine, is pivotal however, there has been no evidence that these processes could be functionally coupled. Using co-immunopurification and spectral shift assays, we found that in the cyanobacterium Synechocystis sp. PCC 6803, the arginine metabolism-related ArgD and CphB enzymes form protein complexes with Gun4, an essential protein for chlorophyll biosynthesis. Gun4 binds ArgD with high affinity, and the Gun4-ArgD complex accumulates in cells supplementedwith ornithine, a key intermediate of the arginine pathway. Elevated ornithine levels restricted de novo synthesis of tetrapyrroles, which arrested the recovery from nitrogen deficiency. Our data reveal a direct crosstalk between tetrapyrrole biosynthesis and arginine metabolism that highlights the importance of balancing photosynthetic pigment synthesis with nitrogen homeostasis.
Permanent Link: https://hdl.handle.net/11104/0347360