0578275 - MBÚ 2024 RIV US eng J - Článek v odborném periodiku
Kotík, Michael - Kulik, Natalia - Valentová, Kateřina
Flavonoids as Aglycones in Retaining Glycosidase-Catalyzed Reactions: Prospects for Green Chemistry.
Journal of Agricultural and Food Chemistry. Roč. 71, č. 41 (2023), s. 14890-14910. ISSN 0021-8561. E-ISSN 1520-5118
Grant CEP: GA ČR(CZ) GA19-00091S; GA ČR(CZ) GA23-04655S
Institucionální podpora: RVO:61388971
Klíčová slova: carnation dianthus-caryophyllus * hydrolyzing beta-glucosidase * active-site mutants * isoflavone-glycosides * substrate-specificity * crystal-structure * enzymatic deglycosylation * thermotoga-neapolitana * aspergillus-oryzae * ms/ms method * Glycoside hydrolase * Hydrolysis * Transglycosylation * Glycosyl donor * Glycosynthase * Glucosidase * Rutinosidase
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 5.7, rok: 2023 ; AIS: 0.903, rok: 2023
Způsob publikování: Open access
Web výsledku:
https://pubs.acs.org/doi/10.1021/acs.jafc.3c04389DOI: https://doi.org/10.1021/acs.jafc.3c04389

Flavonoids and their glycosides are abundant in many plant-based foods. The (de)glycosylation of flavonoids by retaining glycoside hydrolases has recently attracted much interest in basic and applied research, including the possibility of altering the glycosylation pattern of flavonoids. Research in this area is driven by significant differences in physicochemical, organoleptic, and bioactive properties between flavonoid aglycones and their glycosylated counterparts. While many flavonoid glycosides are present in nature at low levels, some occur in substantial quantities, making them readily available low-cost glycosyl donors for transglycosylations. Retaining glycosidases can be used to synthesize natural and novel glycosides, which serve as standards for bioactivity experiments and analyses, using flavonoid glycosides as glycosyl donors. Engineered glycosidases also prove valuable for the synthesis of flavonoid glycosides using chemically synthesized activated glycosyl donors. This review outlines the bioactivities of flavonoids and their glycosides and highlights the applications of retaining glycosidases in the context of flavonoid glycosides, acting as substrates, products, or glycosyl donors in deglycosylation or transglycosylation reactions.
Trvalý link: https://hdl.handle.net/11104/0347295