Reinvestigation of the automated synthesis of stoichiometrically conjugated antibodies to access high molecular weight payloads and multiplexed conjugation via an in-solution trans-tagging process

0578211 - ÚMCH 2024 RIV US eng J - Journal Article
Lehot, V. - Lidický, Ondřej - Most, J. - Erb, S. - Dovgan, I. - Osypenko, A. - Koniev, O. - Kolodych, S. - Kotrchová, Lenka - Chaubet, G. - Cianférani, S. - Etrych, Tomáš - Wagner, A.
Reinvestigation of the automated synthesis of stoichiometrically conjugated antibodies to access high molecular weight payloads and multiplexed conjugation via an in-solution trans-tagging process.
ACS Omega. Roč. 8, č. 43 (2023), s. 40508-40516. ISSN 2470-1343. E-ISSN 2470-1343
R&D Projects: GA MŠMT LX22NPO5102
Institutional support: RVO:61389013
Keywords : protein * antibodies * conjugation
OECD category: Polymer science
Impact factor: 4.1, year: 2022
Method of publishing: Open access
https://pubs.acs.org/doi/10.1021/acsomega.3c05206

Protein conjugates have found applications in a wide variety of fields, ranging from therapeutics to imaging and detection. However, robust control over the parameters of the conjugation process (such as sites and degree of conjugation) remains challenging. Previously, our group introduced Equimolar NAtive Chemical Tagging (ENACT), a method which allows for the monofunctionalization of proteins by combining an iterative low-conversion bioconjugation, an automated process, and a bioorthogonal trans-tagging reaction. However, while the automated ENACT was dimensioned to achieve monoconjugation at the mg scale, in early stage research, because of the rarity and cost of the starting materials, it is often necessary to prepare conjugates at the lower, μg, scale. Here, we introduce modified ENACT protocols, as well as a new ENACT conjugation reagent, which allow for the monofunctionalization of proteins on the micrograms scale, using minimal quantities of payload.
Permanent Link: https://hdl.handle.net/11104/0347570