Offline preparative separation methods based on electromigration: An overview and current trends

0577924 - ÚIACH 2025 RIV NL eng J - Journal Article
Hrušková, Helena - Řemínek, Roman - Foret, František
Offline preparative separation methods based on electromigration: An overview and current trends.
TrAC-Trends in Analytical Chemistry. Roč. 170, JAN (2024), s. 1-9, č. článku 117428. ISSN 0165-9936. E-ISSN 1879-3142
R&D Projects: GA TA ČR(CZ) FW02020209; GA MŠMT EF16_026/0008446
Institutional support: RVO:68081715
Keywords : free-flow electrophoresis * isoelectric focusing * isotachophoresis
OECD category: Analytical chemistry
Impact factor: 13.1, year: 2022
Method of publishing: Open access

Sample preparation is undoubtedly one of the most essential steps in the analytical process. Generally, it facilitates or even enables subsequent measurement of the analytical signal. Electromigration techniques can be effectively used for the preparation of charged analytes. For these reasons, this review article summarizes the progress in offline electromigration preparative techniques between 2018 and 2023. The introduction includes a brief history of the development of electromigration preparative techniques and general theoretical insights into this area of knowledge. The subsequent text consists of four subchapters devoted one by one to electrophoresis, free-flow electrophoresis, isoelectric focusing, and isotachophoresis. Every part includes brief theoretical aspects of the specific electromigration technique and a discussion of the trends, developments, problems, and recently published studies providing a solution for diminishing these limitations.
Permanent Link: https://hdl.handle.net/11104/0347003