Development of the miniaturized instrumentation for caspase activity detection

0577675 - ÚIACH 2024 CZ eng A - Abstract
Killinger, Michael - Veselá, B. - Matalová, E. - Klepárník, Karel
Development of the miniaturized instrumentation for caspase activity detection.
CECE 2023. 18th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2023. s. 60-60. ISBN 978-80-908154-0-7.
[CECE 2023. International Interdisciplinary Meeting on Bioanalysis /18./. 23.10.2023-25.10.2023, Brno]
Institutional support: RVO:68081715
Keywords : miniaturized instrumentation * bioluminescence detection * single-cell analysis
OECD category: Analytical chemistry
https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf

Cell cultures provide important information about the mechanisms of hard tissue development, regeneration, and disease by using bulk analysis of average protein levels in cell populations. However, the cell heterogeneity at the single-cell level has been recognized to be vital for an understanding of various life processes during animal development and disease progress. In this study, we are focused on caspases, the cysteine proteases, which are traditionally associated with cell death via apoptosis but have recently been discovered as important factors in hard tissue differentiation.
Permanent Link: https://hdl.handle.net/11104/0346804