Preparative gel electrophoresis for the fractionation of DNA

0577651 - ÚIACH 2024 CZ eng A - Abstract
Hrušková, Helena - Řemínek, Roman - Foret, František
Preparative gel electrophoresis for the fractionation of DNA.
CECE 2023. 18th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2023. s. 56-56. ISBN 978-80-908154-0-7.
[CECE 2023. International Interdisciplinary Meeting on Bioanalysis /18./. 23.10.2023-25.10.2023, Brno]
R&D Projects: GA TA ČR(CZ) FW02020209
Institutional support: RVO:68081715
Keywords : electrophoresis * DNA fractionation * 3D-printed device
OECD category: Analytical chemistry
https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf

In our study, we developed a new 3D-printed device for DNA fractionation. Using this device, we optimized and partially validated a gel electrophoretic method for the preparative DNA separation. The recovery was ranging between 69-80% and repeatability between 20-31% (RSD, n=48). We also tested the method on the DNA spiked samples of blood serum and blood plasma. In this case, we achieved recovery of 48% and 60% (n=11).
Permanent Link: https://hdl.handle.net/11104/0346783