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A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition

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    0577615 - ÚIACH 2024 CZ eng A - Abstract
    Melicherová, Natália - Gloser, V. - Skalák, J. - Trtílek, M. - Lavická, Jana - Foret, František … Total 7 authors
    A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition.
    CECE 2023. 18th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2023. s. 37-37. ISBN 978-80-908154-0-7.
    [CECE 2023. International Interdisciplinary Meeting on Bioanalysis /18./. 23.10.2023-25.10.2023, Brno]
    R&D Projects: GA MŠMT EF16_026/0008446
    Institutional support: RVO:68081715
    Keywords : capillary electrophoresis * plant sap * sampling * stress * xylem
    OECD category: Analytical chemistry
    https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf

    In this work, an online sampling of plants combined with an efficient capillary electrophoresis (CE)-based method was developed and applied to study the kinetics of changes in the xylem sap composition and to assess plant fitness under stress conditions comprehensively [1,2]. A laboratory-built CE device was developed to provide online sampling and CE analysis of various ionogenic species in the plant sap during plant stress response. The short CE analysis time allows for capturing time-dependent kinetic changes in the living plant during the stress response. The developed device successfully monitored chloride, nitrate, and sulfate ions in the plant xylem during the salt stress or stress caused by nitrate deficiency.
    Permanent Link: https://hdl.handle.net/11104/0346741

     
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