Epitachophoresis - Stabilization of the LE/TE Border

0577562 - ÚIACH 2024 CZ eng A - Abstract
Kocianová, Vanda - Voráčová, Ivona - Foret, František
Epitachophoresis - Stabilization of the LE/TE Border.
CECE 2023. 18th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2023. s. 40-40. ISBN 978-80-908154-0-7.
[CECE 2023. International Interdisciplinary Meeting on Bioanalysis /18./. 23.10.2023-25.10.2023, Brno]
Institutional support: RVO:68081715
Keywords : epitachophoresis * stabilizing medium * DNA concetration
OECD category: Analytical chemistry
https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf

The object of this work is the development and optimization of the epitachophoresis process for the new device design named mini ETP, especially the selection of a medium stabilizing LE/TE border. Different materials for the stabilization of this border were tested, including agarose gels, various foamed polymers, 3D printed structures, and sponges. Due to the problems with the hydrophobicity of some of these materials and analyte sorption, different surface modifications were tried out, including oxygen plasma and surfactants (MHEC, PEG, TRITON X100).
Although the mini ETP concentrates DNA from smaller amounts of the sample (units of mL), it enables a parallel run of multiple experiments. As our future focus is on the long DNA fragments, the “open” structure materials with minimum sieving effect were searched for stabilization of the buffer system during the ETP run. The most promising stabilizing medium from tested options appeared to be a 3D-printed star insert filled with 0.2% agarose gel and the PE hydrophilic disk. The DNA concentration efficiency and recovery were determined to be up to 90%.
Permanent Link: https://hdl.handle.net/11104/0346680