Identification of staphyloxanthin and derivates in yellow-pigmented Staphylococcus capitis subsp. capitis

0577394 - ÚPT 2024 RIV CH eng J - Journal Article
Siems, K. - Runzheimer, K. - Rebrošová, K. - Etzbach, L. - Auerhammer, A. - Rehm, A. - Schwengers, O. - Šiler, Martin - Samek, Ota - Růžička, F. - Moeller, R.
Identification of staphyloxanthin and derivates in yellow-pigmented Staphylococcus capitis subsp. capitis.
Frontiers in Microbiology. Roč. 14, 29 September (2023), č. článku 1272734. ISSN 1664-302X. E-ISSN 1664-302X
R&D Projects: GA MZd(CZ) NU21-05-00341
Institutional support: RVO:68081731
Keywords : Staphylococcus capitis * staphyloxanthin * coagulase-negative staphylococci (CoNS) * carotenoids * bacterial pigments
OECD category: Microbiology
Impact factor: 5.2, year: 2022
Method of publishing: Open access
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1272734/full

Introduction Staphylococcus capitis naturally colonizes the human skin but as an opportunistic pathogen, it can also cause biofilm-associated infections and bloodstream infections in newborns. Previously, we found that two strains from the subspecies S. capitis subsp. capitis produce yellow carotenoids despite the initial species description, reporting this subspecies as non-pigmented. In Staphylococcus aureus, the golden pigment staphyloxanthin is an important virulence factor, protecting cells against reactive oxygen species and modulating membrane fluidity.Methods In this study, we used two pigmented (DSM 111179 and DSM 113836) and two non-pigmented S. capitis subsp. capitis strains (DSM 20326T and DSM 31028) to identify the pigment, determine conditions under which pigment-production occurs and investigate whether pigmented strains show increased resistance to ROS and temperature stress.Results We found that the non-pigmented strains remained colorless regardless of the type of medium, whereas intensity of pigmentation in the two pigmented strains increased under low nutrient conditions and with longer incubation times. We were able to detect and identify staphyloxanthin and its derivates in the two pigmented strains but found that methanol cell extracts from all four strains showed ROS scavenging activity regardless of staphyloxanthin production. Increased survival to cold temperatures (-20 degrees C) was detected in the two pigmented strains only after long-term storage compared to the non-pigmented strains.Conclusion The identification of staphyloxanthin in S. capitis is of clinical relevance and could be used, in the same way as in S. aureus, as a possible target for anti-virulence drug design.
Permanent Link: https://hdl.handle.net/11104/0348042