Negative regulation of MurZ and MurA underlies the essentiality of GpsB- and StkP-mediated protein phosphorylation in iStreptococcus pneumoniae/i D39

0577010 - MBÚ 2024 RIV GB eng J - Journal Article
Tsui, H. T. - Joseph, M. - Zheng, Jiaqi J. J. - Perez, A. J. J. - Manzoor, I. - Rued, Britta E. E. - Richardson, J. D. D. - Branny, Pavel - Doubravová, Linda - Massidda, O. - Winkler, M. E. E.
Negative regulation of MurZ and MurA underlies the essentiality of GpsB- and StkP-mediated protein phosphorylation in iStreptococcus pneumoniae/i D39.
Molecular Microbiology. Roč. 120, č. 3 (2023), s. 351-383. ISSN 0950-382X. E-ISSN 1365-2958
R&D Projects: GA ČR GA18-07748S; GA ČR(CZ) GA19-03269S; GA MŠMT(CZ) LTAUSA18112
Institutional support: RVO:61388971
Keywords : gene duplication and amplification * GpsB peptidoglycan regulator * KhpA/B RNA binding protein * peptidoglycan precursor synthesis * StkP protein kinase
OECD category: Microbiology
Impact factor: 3.6, year: 2022
Method of publishing: Open access
https://onlinelibrary.wiley.com/doi/10.1111/mmi.15122

GpsB links peptidoglycan synthases to other proteins that determine the shape of the respiratory pathogen Streptococcus pneumoniae (pneumococcus, Spn) and other low-GC Gram-positive bacteria. GpsB is also required for phosphorylation of proteins by the essential StkP(Spn) Ser/Thr protein kinase. Here we report three classes of frequently arising chromosomal duplications (approximate to 21-176 genes) containing murZ (MurZ-family homolog of MurA) or murA that suppress Delta gpsB or Delta StkP. These duplications arose from three different repeated sequences and demonstrate the facility of pneumococcus to modulate gene dosage of numerous genes. Overproduction of MurZ or MurA alone or overproduction of MurZ caused by Delta khpAB mutations suppressed Delta gpsB or Delta stkP phenotypes to varying extents. Delta gpsB and Delta stkP were also suppressed by MurZ amino-acid changes distant from the active site, including one in commonly studied laboratory strains, and by truncation or deletion of the homolog of IreB(ReoM). Unlike in other Gram-positive bacteria, MurZ is predominant to MurA in pneumococcal cells. However,Delta gpsB and Delta stkP were not suppressed by Delta clpCP, which did not alter MurZ or MurA amounts. These results support a model in which regulation of MurZ and MurA activity, likely by IreB(Spn), is the only essential requirement for StkP-mediated protein phosphorylation in exponentially growing D39 pneumococcal cells.
Permanent Link: https://hdl.handle.net/11104/0347155