Wild rodents harbour high diversity ofi Arthroderma/i

0577009 - MBÚ 2024 RIV NL eng J - Journal Article
Moulíková, Štěpánka - Kolařík, Miroslav - Lorch, J. M. - Kolarczyková, D. - Hubka, Vít - Čmoková, Adéla
Wild rodents harbour high diversity ofi Arthroderma/i.
Persoonia. Roč. 50, June 2023 (2023), s. 27-47. ISSN 0031-5850
R&D Projects: GA MZd(CZ) NU21-05-00681
Institutional support: RVO:61388971
Keywords : Arthroderma * geophilic dermatophytes * GlobalFungi * mating type genes * new taxa * polyphasic taxonomy * wild rodents
OECD category: Microbiology
Impact factor: 9.1, year: 2022
Method of publishing: Open access
https://www.ingentaconnect.com/content/nhn/pimj/2023/00000050/00000001/art00002

Arthroderma is the most diverse genus of dermatophytes, and its natural reservoir is considered to be soil enriched by keratin sources. During a study on the diversity of dermatophytes in wild small rodents in the Czech Republic, we isolated several strains of Arthroderma. To explore the diversity and ecological significance of these isolates from rodents (n = 29), we characterised the strains genetically (i.e., sequenced ITS, tubb and tef1 & alpha,), mor-phologically, physiologically, and by conducting mating experiments. We then compared the rodent-derived strains to existing ITS sequence data from GenBank and the GlobalFungi Database to further investigate biogeography and the association of Arthroderma species with different types of environments. In total, eight Arthroderma spe-cies were isolated from rodents, including four previously described species (A. crocatum, A. cuniculi, A. curreyi, A. quadrifidum) and four new species proposed herein, i.e., A. rodenticum, A. simile, A. zoogenum and A. psychro- philum. The geographical distribution of these newly described species was not restricted to the Czech Republic nor rodents. Additional isolates were obtained from bats and other mammals, reptiles, and soil from Europe, North America, and Asia. Data mining showed that the genus has a diverse ecology, with some lineages occurring rela-tively frequently in soil, whereas others appeared to be more closely associated with live animals, as we observed in A. rodenticum. Low numbers of sequence reads ascribed to Arthroderma in soil show that the genus is rare in this environment, which supports the hypothesis that Arthroderma spp. are not soil generalists but rather strongly associated with animals and keratin debris. This is the first study to utilise existing metabarcoding data to assess biogeographical, ecological, and diversity patterns in dermatophytes.
Permanent Link: https://hdl.handle.net/11104/0347169