Extracellular vesicles secreted by Echinococcus multilocularis: important players in angiogenesis promotion

0576966 - BC 2024 RIV NL eng J - Journal Article
Liu, C. - Cao, J. - Zhang, H. - Field, Mark Christian - Yin, J.
Extracellular vesicles secreted by Echinococcus multilocularis: important players in angiogenesis promotion.
Microbes and Infection. Roč. 25, č. 7 (2023), č. článku 105147. ISSN 1286-4579. E-ISSN 1769-714X
EU Projects: Wellcome Trust(GB) 204697/Z/16/Z
Institutional support: RVO:60077344
Keywords : Echinococcus multilocularis * Protoscoleces * Extracellular vesicle * Exosome * Proteomics * Angiogenesis
OECD category: Immunology
Impact factor: 5.8, year: 2022
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S1286457923000503?via%3Dihub

The involvement of Echinococcus multilocularis, and other parasitic helminths, in regulating host physi-ology is well recognized, but molecular mechanisms remain unclear. Extracellular vesicles (EVs) released by helminths play important roles in regulating parasite-host interactions by transferring materials to the host. Analysis of protein cargo of EVs from E. multilocularis protoscoleces in the present study revealed a unique composition exclusively associated with vesicle biogenesis. Common proteins in various Echinococcus species were identified, including the classical EVs markers tetraspanins, TSG101 and Alix. Further, unique tegumental antigens were identified which could be exploited as Echinococcus EV markers. Parasite-and host-derived proteins within these EVs are predicted to support important roles in parasite-parasite and parasite-host communication. In addition, the enriched host-derived protein payloads identified in parasite EVs in the present study suggested that they can be involved in focal adhesion and potentially promote angiogenesis. Further, increased angiogenesis was observed in livers of mice infected with E. multilocularis and the expression of several angiogenesis-regulated mol-ecules, including VEGF, MMP9, MCP-1, SDF-1 and serpin E1 were increased. Significantly, EVs released by the E. multilocularis protoscolex promoted proliferation and tube formation by human umbilical vein endothelial cells (HUVECs) in vitro. Taken together, we present the first evidence that tapeworm-secreted EVs may promote angiogenesis in Echinococcus-infections, identifying central mechanisms of Echino-coccus-host interactions.(c) 2023 The Authors. Published by Elsevier Masson SAS on behalf of Institut Pasteur. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Permanent Link: https://hdl.handle.net/11104/0346789