Application of capillary electrophoresis in roughened fused silica capillary for classification of clinical Cutibacterium acnes isolates

0576390 - ÚIACH 2024 IT eng A - Abstract
Šalplachta, Jiří - Horká, Marie - Růžička, F. - Karásek, Pavel - Siváková, A. - Roth, Michal … Total 7 authors
Application of capillary electrophoresis in roughened fused silica capillary for classification of clinical Cutibacterium acnes isolates.
ITP 2023. Abstracts. 2023. s. 142-142.
[International Symposium on Electro- and Liquid-Phase Separation Techniques /29./ ITP 2023. 13.09.2023-17.09.2023, San Felice Circeo]
R&D Projects: GA MZd(CZ) NU22-05-00110
Institutional support: RVO:68081715
Keywords : capillary electrophoresis * classification * cutibacterium acne * fused silica capillary * supercritical water
OECD category: Analytical chemistry

Cutibacterium acnes, commensal bacterium involved in the maintenance of healthy skin, is also associated with inflammatory diseases. Inflammatory and immunogenic properties vary between C. acnes phylotypes, therefore, reliable classification of clinical C. acnes isolates is important for determining their pathogenicity. In this study, a combination of capillary electrophoretic methods, polymer-enhanced transient sotachophoresis and sweeping of the charged bacterial cells in micellar electrokinetic chromatography in the roughened fused silica capillary, was used for the classification of clinical C. acnes isolates into the individual phylotypes. The analysis was done in twenty minutes at laboratory temperature. Separation of the individual isolates of some phylotype groups was achieved by decreasing the separation temperature to 15 °C. Relative standard deviations of migration times of both intra- and inter-day analyses did not exceed 1.7 % and the very good linearity of the proposed method in the concentration range from 5×105 to 1×107 cells mL-1 was achieved (coefficient of determination R2=0.9985). Limit of detection for all the examined bacteria was determined as 5×105 cells mL-1.
Permanent Link: https://hdl.handle.net/11104/0345957