The use of hybrid electrochemical double-stranded DNA/2'-deoxyguanosine 5'-monophosphate (bio)sensors based on anodically pre-treated “basal-plane” pyrolytic graphite electrode for monitoring of the interaction between double-stranded DNA and neonicotinoid insecticide clothianidin

0575985 - FZÚ 2024 RIV GB eng J - Journal Article
Augustín, Michal - Barek, J. - Vyskočil, V.
The use of hybrid electrochemical double-stranded DNA/2'-deoxyguanosine 5'-monophosphate (bio)sensors based on anodically pre-treated “basal-plane” pyrolytic graphite electrode for monitoring of the interaction between double-stranded DNA and neonicotinoid insecticide clothianidin.
Electrochimica acta. Roč. 465, Oct. (2023), č. článku 142972. ISSN 0013-4686. E-ISSN 1873-3859
Institutional support: RVO:68378271
Keywords : biosensor * clothianidin * DNA * intercalation * voltammetry
OECD category: Analytical chemistry
Impact factor: 6.6, year: 2022
Method of publishing: Limited access
https://doi.org/10.1016/j.electacta.2023.142972

We present the utilization of the novel hybrid electrochemical DNA biosensor, as well as the fabrication and testing of novel dGMP (2′-deoxyguanosine 5′-monophosphate) (bio)sensor based on anodically pre-treated “basal-plane” pyrolytic graphite electrode (BPPGE) for the investigation of the interaction between double-stranded DNA (dsDNA) and neonicotinoid insecticide clothianidin (CLOT) via linear-sweep voltammetry (LSV). The results clarify the nature of the interaction as partial-intercalation (marked by high preference towards guanine bases) with binding constant (Kb) of 8.1 × 105 L/mol, free Gibbs energy (∆G0) of −33.154 kJ/mol, and site-binding size (s) of 0.58 bp. The conclusions obtained by LSV are further supported by molecular docking that confirmed a partial-intercalative mode of interaction with the predominant involvement of the hydrogen bonds (exclusively with the guanine bases) for the stabilization of dsDNA-CLOT complex.
Permanent Link: https://hdl.handle.net/11104/0349664