Characterization of newly synthesized labels for analysis of oligosaccharides and glycans by LC/FLD-MS

0575804 - ÚIACH 2024 CZ eng A - Abstract
Smolková, Denisa - Čmelík, Richard - Greguš, M. - Veselý, H. - Pizová, H. - Bobáľ, P. - Lavická, Jana
Characterization of newly synthesized labels for analysis of oligosaccharides and glycans by LC/FLD-MS.
Abstrakty přednášek v sekci Mládí vpřed a plakátových sdělení 24. ročníku Školy hmotnostní spektrometrie. Brno: Spektroskopická společnost Jana Marka Marci, 2023 - (Cvačka, J.; Vrkoslav, V.; Hubálek, M.; Vlk, M.). s. 50-50. ISBN 978-80-88195-43-6.
[Škola hmotnostní spektrometrie /24./. 10.09.2023-15.09.2023, Milovy]
R&D Projects: GA ČR(CZ) GA22-00236S
Institutional support: RVO:68081715
Keywords : LC/FLD-MS analysis * oligosaccharides
OECD category: Analytical chemistry
http://skolams2023.spektroskopie.cz/files/abstrakty_sbornik_SkolaMS2023.pdf

In this work, we present the synthesis of a new fluorescent label suitable for oligosaccharides and glycan analysis by LC with fluorescence and MS detection (LC/FLD-MS). The novel hydrazide derivative of dipyrrometheneboron difluoride (BODIPY) has been synthesized from 2,4-dimethylpyrrole, methyl 4-chloro-4-oxobutanoate and boron trifluoride etherate followed by hydrazinolysis. The novel synthesized label was characterized by several analytical methods including UV/Vis and fluorescence spectroscopy, and MS. Derivatization of oligosaccharides and N-linked glycans by novel fluorescent label was performed via hydrazone formation chemistry under mild conditions followed by LC/FLD-MS analysis.

Permanent Link: https://hdl.handle.net/11104/0345519