Tuning polymer-blood and polymer-cytoplasm membrane interactions by manipulating the architecture of poly(2-oxazoline) triblock copolymers

0575786 - ÚMCH 2024 RIV NL eng J - Journal Article
Lobaz, Volodymyr - Liščáková, Veronika - Sedlák, František - Musil, Dominik - Lukáš Petrova, Svetlana - Šeděnková, Ivana - Pánek, Jiří - Kučka, Jan - Konefal, Rafal - Tihlaříková, Eva - Neděla, Vilém - Pankrác, J. - Šefc, L. - Hrubý, Martin - Šácha, Pavel - Štěpánek, Petr
Tuning polymer-blood and polymer-cytoplasm membrane interactions by manipulating the architecture of poly(2-oxazoline) triblock copolymers.
Colloids and Surfaces B-Biointerfaces. Roč. 231, November (2023), č. článku 113564. ISSN 0927-7765. E-ISSN 1873-4367
R&D Projects: GA MZd(CZ) NU22-03-00318; GA ČR(CZ) GA21-04166S; GA ČR(CZ) GA22-25799S; GA MŠMT LX22NPO5102; GA MŠMT(CZ) LM2023053
Institutional support: RVO:61389013 ; RVO:61388963 ; RVO:68081731
Keywords : amphiphilic triblock polyoxazoline * drug delivery * blood proteins
OECD category: Polymer science; Biochemistry and molecular biology (UOCHB-X); Electrical and electronic engineering (UPT-D)
Impact factor: 5.8, year: 2022
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S0927776523004423?via%3Dihub

Bioactive moieties designed to bind to cell membrane receptors benefit from coupling with polymeric carriers that have enhanced affinity to the cell membrane. When bound to the cell surface, such carriers create a “2D solution” of a ligand with a significantly increased concentration near a membrane-bound receptor compared to a freely water-soluble ligand. Bifunctional polymeric carriers based on amphiphilic triblock copolymers were synthesized from 2-pent-4-ynyl oxazoline, 2-nonyl oxazoline and 2-ethyl oxazoline. Their self-assembly and interactions with plasma proteins and HEK 293 cells were studied in detail. The affinity of these triblock copolymers to HEK 293 cell membranes and organ tissues was tunable by the overall hydrophobicity of the polymer molecule, which is determined by the length of the hydrophobic and hydrophilic blocks. The circulation time and biodistribution of three representative triblock copolymers were monitored after intravenous administration to C57BL/6 albino mice. A prolonged circulation time was observed for polymers with longer hydrophobic blocks, despite their molecular weight being below the renal threshold.
Permanent Link: https://hdl.handle.net/11104/0345639