TPLATE complex-dependent endocytosis attenuates CLAVATA1 signaling for shoot apical meristem maintenance

0575631 - ÚEB 2024 RIV US eng J - Journal Article
Wang, J. - Jiang, Q. - Pleskot, Roman - Grones, P. - Bahafid, E. - Denay, G. - Galván-Ampudia, C. - Xu, X. - Vandorpe, M. - Mylle, E. - De Smet, I. - Vernoux, T. - Simon, R. S. - Nowack, M.K. - Van Damme, D.
TPLATE complex-dependent endocytosis attenuates CLAVATA1 signaling for shoot apical meristem maintenance.
Embo Reports. Roč. 24, č. 9 (2023), č. článku e54709. ISSN 1469-221X. E-ISSN 1469-3178
Institutional support: RVO:61389030
Keywords : clathrin-mediated endocytosis * CLAVATA3-CLAVATA1 signaling * shoot apical meristem maintenance * TPLATE complex
OECD category: Cell biology
Impact factor: 7.7, year: 2022
Method of publishing: Open access
https://doi.org/10.15252/embr.202254709

Endocytosis regulates the turnover of cell surface localized receptors, which are crucial for plants to rapidly respond to stimuli. The evolutionary ancient TPLATE complex (TPC) plays an essential role in endocytosis in Arabidopsis plants. Knockout or knockdown of single TPC subunits causes male sterility and seedling lethality phenotypes, complicating analysis of the roles of TPC during plant development. Partially functional alleles of TPC subunits however only cause mild developmental deviations. Here, we took advantage of the partially functional TPLATE allele, WDXM2, to investigate a role for TPC-dependent endocytosis in receptor-mediated signaling. We discovered that reduced TPC-dependent endocytosis confers a hypersensitivity to very low doses of CLAVATA3 peptide signaling. This hypersensitivity correlated with the abundance of the CLAVATA3 receptor protein kinase CLAVATA1 at the plasma membrane. Genetic and biochemical analysis as well as live-cell imaging revealed that TPC-dependent regulation of CLAVATA3-dependent internalization of CLAVATA1 from the plasma membrane is required for shoot stem cell homeostasis. Our findings provide evidence that TPC-mediated endocytosis and degradation of CLAVATA1 is a mechanism to dampen CLAVATA3-mediated signaling during plant development.
Permanent Link: https://hdl.handle.net/11104/0345375