Domestication has altered the ABA and gibberellin profiles in developing pea seeds

0575458 - ÚEB 2024 RIV DE eng J - Journal Article
Balarynová, J. - Klčová, B. - Tarkowská, Danuše - Turečková, Veronika - Trněný, O. - Špundová, M. - Ochatt, S. - Smýkal, P.
Domestication has altered the ABA and gibberellin profiles in developing pea seeds.
Planta. Roč. 258, č. 2 (2023), č. článku 25. ISSN 0032-0935. E-ISSN 1432-2048
R&D Projects: GA MŠMT(CZ) EF16_019/0000738; GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : Desiccation * Legume * Maturation * Phytohormones * Pigmentation * Seed-coat
OECD category: Biochemistry and molecular biology
Impact factor: 4.3, year: 2022
Method of publishing: Open access
https://doi.org/10.1007/s00425-023-04184-2

Although the role of abscisic acid (ABA) and gibberellins (GAs) interplay has been extensively studied in Arabidopsis and cereals models, comparatively little is known about the effect of domestication on the level of phytohormones in legume seeds. In legumes, as in other crops, seed dormancy has been largely or entirely removed during domestication. In this study, we have measured the endogenous levels of ABA and GAs comparatively between wild and domesticated pea seeds during their development. We have shown that wild seeds contained more ABA than domesticated ones, which could be important for preparing the seeds for the period of dormancy. ABA was catabolised particularly by an 8 & PRIME,-hydroxylation pathway, and dihydrophaseic acid was the main catabolite in seed coats as well as embryos. Besides, the seed coats of wild and pigmented cultivated genotypes were characterised by a broader spectrum of bioactive GAs compared to non-pigmented domesticated seeds. GAs in both seed coat and embryo were synthesized mainly by a 13-hydroxylation pathway, with GA(29) being the most abundant in the seed coat and GA(20) in the embryos. Measuring seed water content and water loss indicated domesticated pea seeds & PRIME, desiccation was slower than that of wild pea seeds. Altogether, we showed that pea domestication led to a change in bioactive GA composition and a lower ABA content during seed development.
Permanent Link: https://hdl.handle.net/11104/0345243