What the Hel: recent advances in understanding rifampicin resistance in bacteria

0575356 - MBÚ 2024 RIV US eng J - Journal Article
Sudzinová, Petra - Šanderová, Hana - Koval, Tomáš - Skálová, Tereza - Borah, Nabajyoti - Hnilicová, Jarmila - Kouba, Tomáš - Dohnálek, Jan - Krásný, Libor
What the Hel: recent advances in understanding rifampicin resistance in bacteria.
FEMS Microbiology Reviews. Roč. 47, č. 6 (2023), č. článku fuac051. ISSN 0168-6445. E-ISSN 1574-6976
R&D Projects: GA ČR(CZ) GA20-12109S; GA ČR(CZ) GA20-07473S; GA MŠMT(CZ) LX22NPO5103; GA MŠMT EF15_003/0000447
Institutional support: RVO:61388971 ; RVO:86652036 ; RVO:61388963
Keywords : rifampicin * antibiotics * resistance * bacteria * RNA polymerase * HelD * HelR
OECD category: Microbiology; Microbiology (BTO-N); Biochemistry and molecular biology (UOCHB-X)
Impact factor: 11.3, year: 2022
Method of publishing: Open access
https://academic.oup.com/femsre/advance-article/doi/10.1093/femsre/fuac051/6957393?login=true

Rifampicin is a clinically important antibiotic that binds to, and blocks the DNA/RNA channel of bacterial RNA polymerase (RNAP). Stalled, nonfunctional RNAPs can be removed from DNA by HelD proteins, this is important for maintenance of genome integrity. Recently, it was reported that HelD proteins from high G+C Actinobacteria, called HelR, are able to dissociate rifampicin-stalled RNAPs from DNA and provide rifampicin resistance. This is achieved by the ability of HelR proteins to dissociate rifampicin from RNAP. The HelR-mediated mechanism of rifampicin resistance is discussed here, and the roles of HelD/HelR in the transcriptional cycle are outlined. Moreover, the possibility that the structurally similar HelD proteins from low G+C Firmicutes may be also involved in rifampicin resistance is explored. Finally, the discovery of the involvement of HelR in rifampicin resistance provides a blueprint for analogous studies to reveal novel mechanisms of bacterial antibiotic resistance.
Permanent Link: https://hdl.handle.net/11104/0345143